Review



mprl  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    R&D Systems mprl
    Mprl, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+prolactin+protein/Recombinant+Mouse+Prolactin+Protein/pmc12107140__pnas%2E2501229122%2Esapp-41-6-7
    Average 93 stars, based on 9 article reviews
    mprl - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Cell Culture:

    Article Title: Ehf controls mammary alveolar lineage differentiation and is a putative suppressor of breast tumorigenesis.
    Article Snippet: .. Organoids were then washed in PBS and cultured in Basal organoid medium containing 0.1% BSA (vehicle control) or Lactation medium containing Advanced DMEM-F12 (Thermo Fisher Scientific) supplemented with 1 mM L-glutamine (Thermofisher Scientific), 100 U/ml Penicillin 100 mg/ml Streptomycin (Thermo Fisher Scientific), 10 mM HEPES (Thermo Fisher Scientific), 5 mg/mL insulin (Symbion), 100 ng/mL hydrocortisone (Symbion) and 5 mg/mL recombinant mouse Prolactin protein (R&D systems) for 96 hours, then harvested for RNA isolation. ..

    Control:

    Article Title: Ehf controls mammary alveolar lineage differentiation and is a putative suppressor of breast tumorigenesis.
    Article Snippet: .. Organoids were then washed in PBS and cultured in Basal organoid medium containing 0.1% BSA (vehicle control) or Lactation medium containing Advanced DMEM-F12 (Thermo Fisher Scientific) supplemented with 1 mM L-glutamine (Thermofisher Scientific), 100 U/ml Penicillin 100 mg/ml Streptomycin (Thermo Fisher Scientific), 10 mM HEPES (Thermo Fisher Scientific), 5 mg/mL insulin (Symbion), 100 ng/mL hydrocortisone (Symbion) and 5 mg/mL recombinant mouse Prolactin protein (R&D systems) for 96 hours, then harvested for RNA isolation. ..

    Recombinant:

    Article Title: Ehf controls mammary alveolar lineage differentiation and is a putative suppressor of breast tumorigenesis.
    Article Snippet: .. Organoids were then washed in PBS and cultured in Basal organoid medium containing 0.1% BSA (vehicle control) or Lactation medium containing Advanced DMEM-F12 (Thermo Fisher Scientific) supplemented with 1 mM L-glutamine (Thermofisher Scientific), 100 U/ml Penicillin 100 mg/ml Streptomycin (Thermo Fisher Scientific), 10 mM HEPES (Thermo Fisher Scientific), 5 mg/mL insulin (Symbion), 100 ng/mL hydrocortisone (Symbion) and 5 mg/mL recombinant mouse Prolactin protein (R&D systems) for 96 hours, then harvested for RNA isolation. ..

    Article Title: KEY RESOURCES TABLE
    Article Snippet: .. Recombinant Mouse Prolactin Protein , R&D Systems , 1445-PL. .. Recombinant Mouse SOD3 Protein , Kusuyama et al. , N/A.

    Article Title: CXCL12-driven thymocyte migration is increased by thymic epithelial cells treated with prolactin in vitro
    Article Snippet: .. Next, the medium was replaced, and the cells were treated with PRL 10 ng/mL (Recombinant Mouse Prolactin Protein; RandD systems, Minneapolis, MN, USA) for 24 h in RPMI medium supplemented with 2% FBS (Carreño et al . ). .. Cell suspensions (1 × 10 6 ) of 2BH4 cells or thymocytes were evaluated for the expression of surface molecules using the following fluorochrome-conjugated antibodies: anti-pan-cytokeratin/Alexa Fluor 488, anti-CD49e/PE, anti-CD49f/FITC (all from eBioscience, San Diego, CA, USA), anti-CD304/APC (Invitrogen), anti-CD184/PE, anti-MHC I/FITC, anti-MHC II/PE, anti-CD4/APC, and anti-CD8/PerCP (all from BD Biosciences, San Diego, CA, USA).

    Isolation:

    Article Title: Ehf controls mammary alveolar lineage differentiation and is a putative suppressor of breast tumorigenesis.
    Article Snippet: .. Organoids were then washed in PBS and cultured in Basal organoid medium containing 0.1% BSA (vehicle control) or Lactation medium containing Advanced DMEM-F12 (Thermo Fisher Scientific) supplemented with 1 mM L-glutamine (Thermofisher Scientific), 100 U/ml Penicillin 100 mg/ml Streptomycin (Thermo Fisher Scientific), 10 mM HEPES (Thermo Fisher Scientific), 5 mg/mL insulin (Symbion), 100 ng/mL hydrocortisone (Symbion) and 5 mg/mL recombinant mouse Prolactin protein (R&D systems) for 96 hours, then harvested for RNA isolation. ..



    Similar Products

    93
    Novus Biologicals mouse prl
    Mouse Prl, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+prolactin+protein/Recombinant+Mouse+Prolactin+Protein/pm40903433-36-25-29
    Average 93 stars, based on 1 article reviews
    mouse prl - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    93
    Novus Biologicals recombinant mouse prl
    Recombinant Mouse Prl, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+prolactin+protein/Recombinant+Mouse+Prolactin+Protein/pm40353577-91-9-15
    Average 93 stars, based on 1 article reviews
    recombinant mouse prl - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    93
    R&D Systems mprl
    Mprl, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+prolactin+protein/Recombinant+Mouse+Prolactin+Protein/pmc12107140__pnas%2E2501229122%2Esapp-41-6-7
    Average 93 stars, based on 1 article reviews
    mprl - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    93
    R&D Systems recombinant mouse prolactin
    (A and G) Time course of prolactin (A) or SOD3 (G) osmotic pump embedding and maternal behavior tests in placental Sod3 KO dam. (B–F and H–L) Plasma levels of prolactin (B and H), number of living pups (C and I), latency of the first retrieval on day 1 (D and J), time spent grooming on day 1 (E and K), and time spent crouching on day 1 (F and L) of WT dams and KO dams with prolactin (B–F) or SOD3 (H–L) osmotic pump embedding of saline or <t>recombinant</t> prolactin. (M–P) mRNA expression levels of prolactin ( Prl ) (M and N) and prolactin receptor ( Prlr ) (O and P) in 200 ng/mL recombinant SOD3-stimulated GH3 cells (M and O) and mouse primary pituitary cells (N and P). N = 5 in each group; three technical replicates for each group; * p < 0.05 and ** p < 0.01.
    Recombinant Mouse Prolactin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+prolactin+protein/Recombinant+Mouse+Prolactin+Protein/pmc11639441-293-8-12
    Average 93 stars, based on 1 article reviews
    recombinant mouse prolactin - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    90
    r&d systems recombinant mouse prolactin protein

    Recombinant Mouse Prolactin Protein, supplied by r&d systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+prolactin+protein/recombinant+human+prolactin/pmc11639441-14-0-5
    Average 90 stars, based on 1 article reviews
    recombinant mouse prolactin protein - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    93
    R&D Systems recombinant proteins recombinant mouse prolactin protein r d systems
    Figure 5. Placental Sod3 KO inhibits the FGF-prolactin axis in the pituitary gland (A) Reactome pathway analysis of placental Sod3f/f and Sod3/ dams. (B) Prl mRNA expression levels of 100 ng/mL <t>recombinant</t> fibroblast growth factor (FGF)1-, FGF2-, or FGF4-stimulated mouse primary pituitary cells with or without 10 mM FGF receptor (FGFR) inhibitors. BGJ398: FGFR1/2/3 inhibitor, PD16686: FGFR1 inhibitor, H3B-6527: FGFR4 inhibitor. N = 5; three technical replicates for each group; **p < 0.01 vs. FGF1-control, yyp < 0.01 vs. FGF2-control, and zzp < 0.01 vs. FGF4-control. (C) Prl mRNA expression levels in recombinant FGF1-, FGF2-, or FGF4-stimulated primary pituitary cells from placental Sod3f/f and Sod3/ dams on day 10 after delivery. (D) Phosphorylation levels of FGFR-induced signaling molecules in recombinant FGF1-, FGF2-, or FGF4-stimulated primary pituitary cells from placental Sod3f/f
    Recombinant Proteins Recombinant Mouse Prolactin Protein R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+prolactin+protein/Recombinant+Mouse+Prolactin+Protein/pm39325622-202-67-73
    Average 93 stars, based on 1 article reviews
    recombinant proteins recombinant mouse prolactin protein r d systems - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    92
    R&D Systems recombinant mouse sod3 protein kusuyama
    Figure 5. Placental <t>Sod3</t> KO inhibits the FGF-prolactin axis in the pituitary gland (A) Reactome pathway analysis of placental Sod3f/f and Sod3/ dams. (B) Prl mRNA expression levels of 100 ng/mL <t>recombinant</t> fibroblast growth factor (FGF)1-, FGF2-, or FGF4-stimulated mouse primary pituitary cells with or without 10 mM FGF receptor (FGFR) inhibitors. BGJ398: FGFR1/2/3 inhibitor, PD16686: FGFR1 inhibitor, H3B-6527: FGFR4 inhibitor. N = 5; three technical replicates for each group; **p < 0.01 vs. FGF1-control, yyp < 0.01 vs. FGF2-control, and zzp < 0.01 vs. FGF4-control. (C) Prl mRNA expression levels in recombinant FGF1-, FGF2-, or FGF4-stimulated primary pituitary cells from placental Sod3f/f and Sod3/ dams on day 10 after delivery. (D) Phosphorylation levels of FGFR-induced signaling molecules in recombinant FGF1-, FGF2-, or FGF4-stimulated primary pituitary cells from placental Sod3f/f
    Recombinant Mouse Sod3 Protein Kusuyama, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+prolactin+protein/Recombinant+Mouse+Prolactin+Protein%2C+CF/pm39325622-202-76-92
    Average 92 stars, based on 1 article reviews
    recombinant mouse sod3 protein kusuyama - by Bioz Stars, 2026-09
    92/100 stars
      Buy from Supplier

    93
    R&D Systems recombinant mouse prolactin protein
    Figure 5. Placental <t>Sod3</t> KO inhibits the FGF-prolactin axis in the pituitary gland (A) Reactome pathway analysis of placental Sod3f/f and Sod3/ dams. (B) Prl mRNA expression levels of 100 ng/mL <t>recombinant</t> fibroblast growth factor (FGF)1-, FGF2-, or FGF4-stimulated mouse primary pituitary cells with or without 10 mM FGF receptor (FGFR) inhibitors. BGJ398: FGFR1/2/3 inhibitor, PD16686: FGFR1 inhibitor, H3B-6527: FGFR4 inhibitor. N = 5; three technical replicates for each group; **p < 0.01 vs. FGF1-control, yyp < 0.01 vs. FGF2-control, and zzp < 0.01 vs. FGF4-control. (C) Prl mRNA expression levels in recombinant FGF1-, FGF2-, or FGF4-stimulated primary pituitary cells from placental Sod3f/f and Sod3/ dams on day 10 after delivery. (D) Phosphorylation levels of FGFR-induced signaling molecules in recombinant FGF1-, FGF2-, or FGF4-stimulated primary pituitary cells from placental Sod3f/f
    Recombinant Mouse Prolactin Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+prolactin+protein/Recombinant+Mouse+Prolactin+Protein/pm38781975-841-59-63
    Average 93 stars, based on 1 article reviews
    recombinant mouse prolactin protein - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    (A and G) Time course of prolactin (A) or SOD3 (G) osmotic pump embedding and maternal behavior tests in placental Sod3 KO dam. (B–F and H–L) Plasma levels of prolactin (B and H), number of living pups (C and I), latency of the first retrieval on day 1 (D and J), time spent grooming on day 1 (E and K), and time spent crouching on day 1 (F and L) of WT dams and KO dams with prolactin (B–F) or SOD3 (H–L) osmotic pump embedding of saline or recombinant prolactin. (M–P) mRNA expression levels of prolactin ( Prl ) (M and N) and prolactin receptor ( Prlr ) (O and P) in 200 ng/mL recombinant SOD3-stimulated GH3 cells (M and O) and mouse primary pituitary cells (N and P). N = 5 in each group; three technical replicates for each group; * p < 0.05 and ** p < 0.01.

    Journal: Cell reports

    Article Title: Placenta-derived SOD3 deletion impairs maternal behavior via alterations in FGF/FGFR-prolactin signaling axis

    doi: 10.1016/j.celrep.2024.114789

    Figure Lengend Snippet: (A and G) Time course of prolactin (A) or SOD3 (G) osmotic pump embedding and maternal behavior tests in placental Sod3 KO dam. (B–F and H–L) Plasma levels of prolactin (B and H), number of living pups (C and I), latency of the first retrieval on day 1 (D and J), time spent grooming on day 1 (E and K), and time spent crouching on day 1 (F and L) of WT dams and KO dams with prolactin (B–F) or SOD3 (H–L) osmotic pump embedding of saline or recombinant prolactin. (M–P) mRNA expression levels of prolactin ( Prl ) (M and N) and prolactin receptor ( Prlr ) (O and P) in 200 ng/mL recombinant SOD3-stimulated GH3 cells (M and O) and mouse primary pituitary cells (N and P). N = 5 in each group; three technical replicates for each group; * p < 0.05 and ** p < 0.01.

    Article Snippet: Osmotic pumps were filled with 7.5 μg of recombinant mouse prolactin (1445-PL, R&D Systems) or SOD3 diluted in 1 mL of phosphate-buffered saline (PBS).

    Techniques: Clinical Proteomics, Saline, Recombinant, Expressing

    (A) Reactome pathway analysis of placental Sod3 f/f and Sod3 −/− dams. (B) Prl mRNA expression levels of 100 ng/mL recombinant fibroblast growth factor (FGF)1-, FGF2-, or FGF4-stimulated mouse primary pituitary cells with or without 10 μM FGF receptor (FGFR) inhibitors. BGJ398: FGFR1/2/3 inhibitor, PD16686: FGFR1 inhibitor, H3B-6527: FGFR4 inhibitor. N = 5; three technical replicates for each group; ** p < 0.01 vs. FGF1-control, †† p < 0.01 vs. FGF2-control, and ‡‡ p < 0.01 vs. FGF4-control. (C) Prl mRNA expression levels in recombinant FGF1-, FGF2-, or FGF4-stimulated primary pituitary cells from placental Sod3 f/f and Sod3 −/− dams on day 10 after delivery. (D) Phosphorylation levels of FGFR-induced signaling molecules in recombinant FGF1-, FGF2-, or FGF4-stimulated primary pituitary cells from placental Sod3 f/f and Sod3 −/− dams. N = 3 in each group; three biological and technical replicates for each group.

    Journal: Cell reports

    Article Title: Placenta-derived SOD3 deletion impairs maternal behavior via alterations in FGF/FGFR-prolactin signaling axis

    doi: 10.1016/j.celrep.2024.114789

    Figure Lengend Snippet: (A) Reactome pathway analysis of placental Sod3 f/f and Sod3 −/− dams. (B) Prl mRNA expression levels of 100 ng/mL recombinant fibroblast growth factor (FGF)1-, FGF2-, or FGF4-stimulated mouse primary pituitary cells with or without 10 μM FGF receptor (FGFR) inhibitors. BGJ398: FGFR1/2/3 inhibitor, PD16686: FGFR1 inhibitor, H3B-6527: FGFR4 inhibitor. N = 5; three technical replicates for each group; ** p < 0.01 vs. FGF1-control, †† p < 0.01 vs. FGF2-control, and ‡‡ p < 0.01 vs. FGF4-control. (C) Prl mRNA expression levels in recombinant FGF1-, FGF2-, or FGF4-stimulated primary pituitary cells from placental Sod3 f/f and Sod3 −/− dams on day 10 after delivery. (D) Phosphorylation levels of FGFR-induced signaling molecules in recombinant FGF1-, FGF2-, or FGF4-stimulated primary pituitary cells from placental Sod3 f/f and Sod3 −/− dams. N = 3 in each group; three biological and technical replicates for each group.

    Article Snippet: Osmotic pumps were filled with 7.5 μg of recombinant mouse prolactin (1445-PL, R&D Systems) or SOD3 diluted in 1 mL of phosphate-buffered saline (PBS).

    Techniques: Expressing, Recombinant, Control, Phospho-proteomics

    Journal: Cell reports

    Article Title: Placenta-derived SOD3 deletion impairs maternal behavior via alterations in FGF/FGFR-prolactin signaling axis

    doi: 10.1016/j.celrep.2024.114789

    Figure Lengend Snippet:

    Article Snippet: Osmotic pumps were filled with 7.5 μg of recombinant mouse prolactin (1445-PL, R&D Systems) or SOD3 diluted in 1 mL of phosphate-buffered saline (PBS).

    Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Catecholamine ELISA, Clinical Proteomics, Sandwich ELISA, Dehydrogenase Assay, Activity Assay, Software

    Journal: Cell reports

    Article Title: Placenta-derived SOD3 deletion impairs maternal behavior via alterations in FGF/FGFR-prolactin signaling axis

    doi: 10.1016/j.celrep.2024.114789

    Figure Lengend Snippet:

    Article Snippet: Recombinant Mouse Prolactin Protein , R&D Systems , 1445-PL.

    Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Catecholamine ELISA, Clinical Proteomics, Sandwich ELISA, Dehydrogenase Assay, Activity Assay, Software

    Figure 5. Placental Sod3 KO inhibits the FGF-prolactin axis in the pituitary gland (A) Reactome pathway analysis of placental Sod3f/f and Sod3/ dams. (B) Prl mRNA expression levels of 100 ng/mL recombinant fibroblast growth factor (FGF)1-, FGF2-, or FGF4-stimulated mouse primary pituitary cells with or without 10 mM FGF receptor (FGFR) inhibitors. BGJ398: FGFR1/2/3 inhibitor, PD16686: FGFR1 inhibitor, H3B-6527: FGFR4 inhibitor. N = 5; three technical replicates for each group; **p < 0.01 vs. FGF1-control, yyp < 0.01 vs. FGF2-control, and zzp < 0.01 vs. FGF4-control. (C) Prl mRNA expression levels in recombinant FGF1-, FGF2-, or FGF4-stimulated primary pituitary cells from placental Sod3f/f and Sod3/ dams on day 10 after delivery. (D) Phosphorylation levels of FGFR-induced signaling molecules in recombinant FGF1-, FGF2-, or FGF4-stimulated primary pituitary cells from placental Sod3f/f

    Journal: Cell reports

    Article Title: Placenta-derived SOD3 deletion impairs maternal behavior via alterations in FGF/FGFR-prolactin signaling axis.

    doi: 10.1016/j.celrep.2024.114789

    Figure Lengend Snippet: Figure 5. Placental Sod3 KO inhibits the FGF-prolactin axis in the pituitary gland (A) Reactome pathway analysis of placental Sod3f/f and Sod3/ dams. (B) Prl mRNA expression levels of 100 ng/mL recombinant fibroblast growth factor (FGF)1-, FGF2-, or FGF4-stimulated mouse primary pituitary cells with or without 10 mM FGF receptor (FGFR) inhibitors. BGJ398: FGFR1/2/3 inhibitor, PD16686: FGFR1 inhibitor, H3B-6527: FGFR4 inhibitor. N = 5; three technical replicates for each group; **p < 0.01 vs. FGF1-control, yyp < 0.01 vs. FGF2-control, and zzp < 0.01 vs. FGF4-control. (C) Prl mRNA expression levels in recombinant FGF1-, FGF2-, or FGF4-stimulated primary pituitary cells from placental Sod3f/f and Sod3/ dams on day 10 after delivery. (D) Phosphorylation levels of FGFR-induced signaling molecules in recombinant FGF1-, FGF2-, or FGF4-stimulated primary pituitary cells from placental Sod3f/f

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FGF1 abcam Cat# ab9588; RRID:AB_308729 FGFR2 Sigma-Aldrich Cat# SAB4500889 phospho-PI3K Cell Signaling Technology Cat# 17366; RRID:AB_2895293 phospho-PKCd Cell Signaling Technology Cat# 2055; RRID:AB_330876 PKCd Cell Signaling Technology Cat# 2058; RRID:AB_10694655 phospho-PLCg1 Cell Signaling Technology Cat# 14008; RRID:AB_2728690 PLCg1 Cell Signaling Technology Cat# 2822; RRID:AB_2163702 bTubulin Cell Signaling Technology Cat# 2128; RRID:AB_823664 5-hmC antibody Active Motif Cat# 39769; RRID:AB_10013602 Chemicals, peptides, and recombinant proteins Recombinant Mouse Prolactin Protein R&D Systems 1445-PL Recombinant Mouse SOD3 Protein Kusuyama et al.15 N/A Cabergoline Cayman Chemical 23934 Recombinant Mouse FGF1 Protein R&D Systems 4686-FA Recombinant Mouse FGF2 Protein Fujifilm Wako 062–05181 Recombinant Mouse FGF4 Protein R&D Systems 5846-F4 BGJ398 Cayman Chemical 19157 PD166866 Cayman Chemical 22464 H3B-6527 Cayman Chemical 26072 Critical commercial assays SOD3 ELISA Kit (Mouse) Aviva System Biology OKCD01107 Mouse Prolactin ELISA Kit abcam ab100736 Oxytocin ELISA Kit Wako Fujifilm Wako 292–84401 Estradiol Parameter Assay Kit R&D Systems KGE014 Mouse progesterone (PROG) ELISA Kit Cusabio CSB-E05104m Corticosterone ELISA kit abcam ab108821 Dopamine ELISA kit – Fast Plasma & Urine samples ImmuSmol BA E 5300R Mouse Placental Lactogen (Sandwich ELISA) ELISA Kit LifeSpan BioSciences LS-F28728-1 Mouse FGF acidic/FGF1 DuoSet ELISA R&D Systems DY4686-05 Mouse FGF basic/FGF2/bFGF DuoSet ELISA R&D Systems DY3139-05 Mouse FGF-4 ELISA Kit Ray Biotech ELM-FGF4-1 Alpha Ketoglutarate (alpha KG) Assay Kit abcam ab83431 Isocitrate Dehydrogenase Assay Kit (Colorimetric) abcam ab102528 TET Hydroxylase Activity Quantification Kit (Fluorometric) abcam ab156913 Experimental models: Cell lines GH3 JCRB Cell Bank JCRB9047 Experimental models: Organisms/strains Sod3f/f mice Kusuyama et al.15 N/A Tpbpa/Ada Cre mice Kusuyama et al.15 N/A Tpbpa/Ada Cre; Sod3f/f mice This paper N/A C57BL/6 Charles River M/A (Continued on next page) Cell Reports 43, 114789, October 22, 2024 17

    Techniques: Expressing, Recombinant, Control, Phospho-proteomics

    Figure 5. Placental Sod3 KO inhibits the FGF-prolactin axis in the pituitary gland (A) Reactome pathway analysis of placental Sod3f/f and Sod3/ dams. (B) Prl mRNA expression levels of 100 ng/mL recombinant fibroblast growth factor (FGF)1-, FGF2-, or FGF4-stimulated mouse primary pituitary cells with or without 10 mM FGF receptor (FGFR) inhibitors. BGJ398: FGFR1/2/3 inhibitor, PD16686: FGFR1 inhibitor, H3B-6527: FGFR4 inhibitor. N = 5; three technical replicates for each group; **p < 0.01 vs. FGF1-control, yyp < 0.01 vs. FGF2-control, and zzp < 0.01 vs. FGF4-control. (C) Prl mRNA expression levels in recombinant FGF1-, FGF2-, or FGF4-stimulated primary pituitary cells from placental Sod3f/f and Sod3/ dams on day 10 after delivery. (D) Phosphorylation levels of FGFR-induced signaling molecules in recombinant FGF1-, FGF2-, or FGF4-stimulated primary pituitary cells from placental Sod3f/f

    Journal: Cell reports

    Article Title: Placenta-derived SOD3 deletion impairs maternal behavior via alterations in FGF/FGFR-prolactin signaling axis.

    doi: 10.1016/j.celrep.2024.114789

    Figure Lengend Snippet: Figure 5. Placental Sod3 KO inhibits the FGF-prolactin axis in the pituitary gland (A) Reactome pathway analysis of placental Sod3f/f and Sod3/ dams. (B) Prl mRNA expression levels of 100 ng/mL recombinant fibroblast growth factor (FGF)1-, FGF2-, or FGF4-stimulated mouse primary pituitary cells with or without 10 mM FGF receptor (FGFR) inhibitors. BGJ398: FGFR1/2/3 inhibitor, PD16686: FGFR1 inhibitor, H3B-6527: FGFR4 inhibitor. N = 5; three technical replicates for each group; **p < 0.01 vs. FGF1-control, yyp < 0.01 vs. FGF2-control, and zzp < 0.01 vs. FGF4-control. (C) Prl mRNA expression levels in recombinant FGF1-, FGF2-, or FGF4-stimulated primary pituitary cells from placental Sod3f/f and Sod3/ dams on day 10 after delivery. (D) Phosphorylation levels of FGFR-induced signaling molecules in recombinant FGF1-, FGF2-, or FGF4-stimulated primary pituitary cells from placental Sod3f/f

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FGF1 abcam Cat# ab9588; RRID:AB_308729 FGFR2 Sigma-Aldrich Cat# SAB4500889 phospho-PI3K Cell Signaling Technology Cat# 17366; RRID:AB_2895293 phospho-PKCd Cell Signaling Technology Cat# 2055; RRID:AB_330876 PKCd Cell Signaling Technology Cat# 2058; RRID:AB_10694655 phospho-PLCg1 Cell Signaling Technology Cat# 14008; RRID:AB_2728690 PLCg1 Cell Signaling Technology Cat# 2822; RRID:AB_2163702 bTubulin Cell Signaling Technology Cat# 2128; RRID:AB_823664 5-hmC antibody Active Motif Cat# 39769; RRID:AB_10013602 Chemicals, peptides, and recombinant proteins Recombinant Mouse Prolactin Protein R&D Systems 1445-PL Recombinant Mouse SOD3 Protein Kusuyama et al.15 N/A Cabergoline Cayman Chemical 23934 Recombinant Mouse FGF1 Protein R&D Systems 4686-FA Recombinant Mouse FGF2 Protein Fujifilm Wako 062–05181 Recombinant Mouse FGF4 Protein R&D Systems 5846-F4 BGJ398 Cayman Chemical 19157 PD166866 Cayman Chemical 22464 H3B-6527 Cayman Chemical 26072 Critical commercial assays SOD3 ELISA Kit (Mouse) Aviva System Biology OKCD01107 Mouse Prolactin ELISA Kit abcam ab100736 Oxytocin ELISA Kit Wako Fujifilm Wako 292–84401 Estradiol Parameter Assay Kit R&D Systems KGE014 Mouse progesterone (PROG) ELISA Kit Cusabio CSB-E05104m Corticosterone ELISA kit abcam ab108821 Dopamine ELISA kit – Fast Plasma & Urine samples ImmuSmol BA E 5300R Mouse Placental Lactogen (Sandwich ELISA) ELISA Kit LifeSpan BioSciences LS-F28728-1 Mouse FGF acidic/FGF1 DuoSet ELISA R&D Systems DY4686-05 Mouse FGF basic/FGF2/bFGF DuoSet ELISA R&D Systems DY3139-05 Mouse FGF-4 ELISA Kit Ray Biotech ELM-FGF4-1 Alpha Ketoglutarate (alpha KG) Assay Kit abcam ab83431 Isocitrate Dehydrogenase Assay Kit (Colorimetric) abcam ab102528 TET Hydroxylase Activity Quantification Kit (Fluorometric) abcam ab156913 Experimental models: Cell lines GH3 JCRB Cell Bank JCRB9047 Experimental models: Organisms/strains Sod3f/f mice Kusuyama et al.15 N/A Tpbpa/Ada Cre mice Kusuyama et al.15 N/A Tpbpa/Ada Cre; Sod3f/f mice This paper N/A C57BL/6 Charles River M/A (Continued on next page) Cell Reports 43, 114789, October 22, 2024 17

    Techniques: Expressing, Recombinant, Control, Phospho-proteomics